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KMID : 0361020140570080526
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Korean Journal of Otolaryngology - Head and Neck Surgery
2014 Volume.57 No. 8 p.526 ~ p.532
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Effect of Betulinic Acid on MUC5AC and MUC5B Expression in Airway Epithelial Cells
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Kim Hoon-Sung
Choi Yoon-Seok
Lee Jun-Hyeok
Park Na-Kyung
Park Chang-Hwi
Lee Young-Ha
Kim Gui-Ok
Song Si-Youn
Bae Chang-Hoon
Lee Seung-Ho
Kim Yong-Woon
Kim Yong-Dae
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Abstract
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Background and Objectives: MUC5AC and MUC5B are representative secretory mucin genes in the human airway, whose expressions are increased by a variety of inflammatory mediators. Betulinic acid, a naturally occurring pentacyclic triterpenoid, is known to have an anti-inflammatory property. However, the effects of betulinic acid on mucin secretion of airway epithelial cells still have not been reported. Therefore, in this study, the effect of betulinic acid on inflammatory mediators-induced MUC5AC and MUC5B expressions was investigated in human airway epithelial cells.
Subjects and Methods: In the mucin-producing human NCI-H292 airway epithelial cells, the effects of betulinic acid on interleukin-1β (IL-1β)-, lipopolysaccharide (LPS)-, and phorbol myristate acetate (PMA)-induced MUC5AC and MUC5B expressions were analyzed by reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay.
Results: Betulinic acid attenuated IL-1β-, LPS-, and PMA-induced MUC5B mRNA and glycoprotein expression in NCI-H292 cells. On the other hand, betulinic acid did not attenuate IL-1β-, and LPS-, but induced PMA-induced MUC5AC mRNA and glycoprotein expressions in NCI-H292 cells.
Conclusion: These results suggest that betulinic acid attenuates IL-1β-, LPS-, and PMA-induced MUC5B expression in the airway epithelial cells. Therefore, betulinic acid may modulate a control of mucus-hypersecretion in airway inflammatory diseases.
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KEYWORD
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Betulinic acid, Human airway epithelial cells, MUC5AC, MUC5B
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